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antibodies detecting glut4  (R&D Systems)


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    R&D Systems antibodies detecting glut4
    Antibodies Detecting Glut4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+detecting+glut4/Glut4+Antibody/pmc04587640-147-11-17
    Average 91 stars, based on 2 article reviews
    antibodies detecting glut4 - by Bioz Stars, 2026-10
    91/100 stars

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    Saline:

    Article Title: Increased Skeletal Muscle Capillarization Independently Enhances Insulin Sensitivity in Older Adults After Exercise Training and Detraining
    Article Snippet: After gel electrophoresis, proteins were transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA) and blocked with 5% nonfat dry milk for 1 h at room temperature. .. Membranes were washed briefly with Tris-buffered saline and incubated with primary antibodies detecting GLUT4 (anti-GLUT4, 5 μg/mL; R&D Systems, Minneapolis, MN), AMPKα1, total Akt, or pAkt Ser473 (anti-AMPKα1, anti-Akt, and anti-pAkt Ser473 , 1:1,000; Cell Signaling Technology, Danvers, MA). β-Actin (anti-β-actin, 1:1,000; Cell Signaling Technology) was used for total protein normalization. .. After primary antibody incubation, membranes were washed with Tris-buffered saline and incubated with horseradish peroxidase–linked anti-rabbit IgG or anti-mouse IgG (1:1,000; Cell Signaling Technology) secondary antibodies for 1 h at room temperature.

    Incubation:

    Article Title: Increased Skeletal Muscle Capillarization Independently Enhances Insulin Sensitivity in Older Adults After Exercise Training and Detraining
    Article Snippet: After gel electrophoresis, proteins were transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA) and blocked with 5% nonfat dry milk for 1 h at room temperature. .. Membranes were washed briefly with Tris-buffered saline and incubated with primary antibodies detecting GLUT4 (anti-GLUT4, 5 μg/mL; R&D Systems, Minneapolis, MN), AMPKα1, total Akt, or pAkt Ser473 (anti-AMPKα1, anti-Akt, and anti-pAkt Ser473 , 1:1,000; Cell Signaling Technology, Danvers, MA). β-Actin (anti-β-actin, 1:1,000; Cell Signaling Technology) was used for total protein normalization. .. After primary antibody incubation, membranes were washed with Tris-buffered saline and incubated with horseradish peroxidase–linked anti-rabbit IgG or anti-mouse IgG (1:1,000; Cell Signaling Technology) secondary antibodies for 1 h at room temperature.



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